Black Widow may refer to:

Wildlife

• The black widow spider, the common name for several species of venomous spider
• False black widow, spiders of the genus Steatoda, often mistaken for widow spiders
• Black widow (fish) (Stygnobrotula latebricola), a species of brotula

Literature

• Black Widow (Marvel Comics), two Marvel Comics characters
• Black Widow (Timely Comics), a 1940s comic book character
• Black Widow: A Novel, a 1981 novel by Christina Crawford
• Black Widowers, a fictional men-only dining club created by Isaac Asimov for a series of mystery stories

Film and television

• Black Widow (1954 film), a film starring Ginger Rogers and Gene Tierney
• Black Widow (1987 film), a film starring Debra Winger and Theresa Russell
• Black Widow (2005 film), a Canadian film starring Sarah Slean
• Black Widow (2007 film), a film starring Elizabeth Berkley and Alicia Coppola
• The Black Widow (Serial), a 1947 movie serial starring Bruce Edwards
• The Black Widow (1951 film), a British film starring Christine Norden
• "The Black Widow" (Boston Legal episode), an episode of Boston Legal
• "Black Widower", an episode of The Simpsons
• Black Widows motorcycle gang, in the Clint Eastwood films Every Which Way But Loose and Any Which Way You Can

Music

• Black Widow (band), a British progressive rock band
• Criteria for a Black Widow, a 2000 album by the heavy metal band Annihilator
• "Black Widow", a song by the rock band Mötley Crüe on the compilation Red, White and Crüe
• "Black Widow", a song by Lita Ford on the album Dangerous Curves
• "Black Widow", a song by the melodic death metal band Children of Bodom on the album Hatebreeder
• "Black Widow", a song by Dolores O'Riordan on the album Are You Listening?
• "The Black Widow", a song by Alice Cooper on the album Welcome to My Nightmare
• Black Widow Records, an Italian heavy metal label

Video and computer games

• Black Widow (arcade game), a 1982 Atari arcade game
• Black Widow (Slam Masters), a character from the Slam Masters series of games by Capcom
• Black Widow Games, a computer games developer

Miscellanea

• wikt:black widow, a human female who has killed one or more of her husband(s).
• Black Widow (truck), a monster truck that races on the USHRA circuit
• Black Widow Spider Bike, a motorcycle featured on the reality TV show American Chopper
• P-61 Black Widow, an American night fighter aircraft used in World War II
• Black Widows, Chechen female suicide bombers
• Black Widow Cheer Gym, an all-star cheerleading facility in Ottawa, Canada
• Blackarachnia, a Transformers character known as Black Widow in Japan
• Northrop YF-23, a prototype fighter aircraft designed for the United States Air Force, nicknamed "Black Widow II"
• Black Widow, the development code name for the Cray X2 supercomputer
• A girl dressed all in black accompanied by a man in a Zoot Suit
• The nickname of Jeanette Lee, a pool player who tends to dress in black
• The nickname of Sonya Thomas, a champion competitive eater
• The name of a paint scheme applied to Southern Pacific railroad diesel locomotives

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